3C8C
Crystal structure of Mcp_N and cache domains of methyl-accepting chemotaxis protein from Vibrio cholerae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2008-02-08 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9796 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 103.202, 140.942, 32.221 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.500 |
R-factor | 0.19495 |
Rwork | 0.194 |
R-free | 0.23452 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.285 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.140 | 1.540 |
High resolution limit [Å] | 1.460 | 1.460 |
Rmerge | 0.108 | 0.920 |
Number of reflections | 81575 | |
<I/σ(I)> | 13.1 | 2.4 |
Completeness [%] | 96.1 | 95.6 |
Redundancy | 13.7 | 11.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 100mM Sodium cacodylate pH 6.5, 200mM Sodium acetate, 30% PEG 8000, 10% Glycerol, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K |