3C83

Bacteriophage T4 lysozyme mutant D89A in wildtype background at room temperature

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1998-01-29
Detector	SDMS
Spacegroup name	P 32 2 1
Unit cell lengths	61.000, 61.000, 97.170
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.220 - 1.840
R-factor	0.183
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l63
RMSD bond length	0.012
RMSD bond angle	1.314
Data reduction software	SAN (DIEGO MULTIWIRE)
Data scaling software	SAN (DEIGO MULTIWIRE)
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.220	1.870
High resolution limit [Å]	1.840	1.840
Rmerge	0.066
Number of reflections	17756
Completeness [%]	95.0	83.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.7		2 M NA/K PHOSPHATE PH 6.7 550 MM NACL 50 MM REDUCED BME, 50 MM OXIDIZED BME , VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 277K, pH 6.70