3C80

T4 Lysozyme mutant R96Y at room temperature

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1998-11-14
Detector	RIGAKU RAXIS II
Spacegroup name	P 32 2 1
Unit cell lengths	60.433, 60.433, 97.033
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.760 - 1.990
R-factor	0.199
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l63
RMSD bond length	0.010
RMSD bond angle	1.216
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.760	2.100
High resolution limit [Å]	1.990	1.990
Rmerge	0.048	0.103
Number of reflections	13030
<I/σ(I)>	27.3	12.8
Completeness [%]	90.2	65.5
Redundancy	6.1	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.7		2 M NA/K PHOSPHATE PH 6.7 550 MM NACL 50 MM REDUCED BME, 50 MM OXIDIZED BME , VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 277K, pH 6.70