3C7U
Structural Insight into the Kinetics and Cp of interactions between TEM-1-Lactamase and BLIP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CAMD BEAMLINE GCPCC |
| Synchrotron site | CAMD |
| Beamline | GCPCC |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.3808 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.090, 129.476, 80.130 |
| Unit cell angles | 90.00, 91.86, 90.00 |
Refinement procedure
| Resolution | 32.410 - 2.200 |
| R-factor | 0.20954 |
| Rwork | 0.208 |
| R-free | 0.23202 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jtg |
| RMSD bond length | 0.026 |
| RMSD bond angle | 1.926 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 32.410 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.065 |
| Number of reflections | 50562 |
| <I/σ(I)> | 12.5 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 15% PEG 8000, 0.1M Phosphate-Citrate, 0.1M NaCl, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
