3C7T
Crystal structure of the ecdysone phosphate phosphatase, EPPase, from Bombix mori in complex with tungstate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-05 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.901, 134.410, 135.188 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.200 - 1.760 |
| R-factor | 0.18201 |
| Rwork | 0.180 |
| R-free | 0.21058 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.389 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.200 | 1.800 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.099 | 0.654 |
| Number of reflections | 114147 | |
| <I/σ(I)> | 29.6 | 2.6 |
| Completeness [%] | 99.7 | 97.8 |
| Redundancy | 6.9 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 16% Peg8000, 0.2 M magnesium chloride, 0.1 M Tris-HCl, 5 mM sodium tungstate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
