3C7M
Crystal structure of reduced DsbL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2007-05-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.8555 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.945, 66.634, 77.891 |
| Unit cell angles | 90.00, 107.02, 90.00 |
Refinement procedure
| Resolution | 19.740 - 1.550 |
| R-factor | 0.16502 |
| Rwork | 0.164 |
| R-free | 0.18892 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ANOTHER CRYSTAL FORM OF DSBL SOLVED BY SAD USING CADMIUM ANOMALOUS SIGNAL |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.997 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (1.3.1) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.650 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 70367 | |
| <I/σ(I)> | 13.7 | 4.3 |
| Completeness [%] | 97.2 | 96.3 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 277 | 0.1 M acetic acid-NaOH pH 4.6, 0.14 M CdCl2, 29% PEG 400, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
