3C7L
Molecular architecture of Galphao and the structural basis for RGS16-mediated deactivation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-10-01 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 1.10 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.379, 71.700, 72.306 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.890 |
| Rwork | 0.222 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1agr |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.021 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.890 |
| Number of reflections | 20395 |
| <I/σ(I)> | 21.3 |
| Completeness [%] | 94.6 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 34% PEG 400 150 mM ammonium citrate 100 mM cacodylate pH 6.5 0.5 mM KCl6Ir(IV), VAPOR DIFFUSION, HANGING DROP, temperature 277K |
