3C75

Paracoccus versutus methylamine dehydrogenase in complex with amicyanin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, Hamburg
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-02-08
Detector	MAR scanner 345 mm plate
Spacegroup name	P 21 21 21
Unit cell lengths	55.573, 131.038, 171.689
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.150 - 2.500
R-factor	0.235
Rwork	0.235
R-free	0.28300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.680
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.143	0.449
Number of reflections	37332
<I/σ(I)>	6.51	2.25
Completeness [%]	84.1	80.9
Redundancy	3.8	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5	293	PEG 8000; lithium sulfate, phosphate buffer, pH 6.50, VAPOR DIFFUSION, SITTING DROP, temperature 293K