3C74

X-ray structure of the uridine phosphorylase from salmonella typhimurium in complex with 2,2'-anhydrouridine at 2.38a resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Detector technology	CCD
Collection date	2006-11-01
Detector	MAR CCD 165 mm
Wavelength(s)	1.0500
Spacegroup name	P 21 21 21
Unit cell lengths	90.370, 125.308, 135.177
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.940 - 2.380
R-factor	0.20944
Rwork	0.208
R-free	0.25787
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.154
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.380
Number of reflections	61088

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	PEG400, NaN3, VAPOR DIFFUSION, HANGING DROP, temperature 297K