3C6P
Small molecule agonists and antagonists of F-box protein-substrate interactions in auxin perception and signaling
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 102.794, 80.386, 125.206 |
| Unit cell angles | 90.00, 104.72, 90.00 |
Refinement procedure
| Resolution | 49.710 - 2.700 |
| R-factor | 0.18454 |
| Rwork | 0.180 |
| R-free | 0.26075 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p1m |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.479 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 24753 | |
| Completeness [%] | 75.0 | 95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6 | 277 | 12-14% PEG 40,000, 100mM BTP pH6.0, 200mM NaCl, EVAPORATION, temperature 277K |
