3C6P
Small molecule agonists and antagonists of F-box protein-substrate interactions in auxin perception and signaling
Experimental procedure
Experimental method | LAUE |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 102.794, 80.386, 125.206 |
Unit cell angles | 90.00, 104.72, 90.00 |
Refinement procedure
Resolution | 49.710 - 2.700 |
R-factor | 0.18454 |
Rwork | 0.180 |
R-free | 0.26075 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p1m |
RMSD bond length | 0.025 |
RMSD bond angle | 2.479 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 24753 | |
Completeness [%] | 75.0 | 95 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6 | 277 | 12-14% PEG 40,000, 100mM BTP pH6.0, 200mM NaCl, EVAPORATION, temperature 277K |