3C6N
Small molecule agonists and antagonists of F-box protein-substrate interactions in auxin perception and signaling
Experimental procedure
Experimental method | LAUE |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-21 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 102.510, 80.661, 125.071 |
Unit cell angles | 90.00, 104.95, 90.00 |
Refinement procedure
Resolution | 49.520 - 2.600 |
R-factor | 0.19 |
Rwork | 0.185 |
R-free | 0.28100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p1m |
RMSD bond length | 0.035 |
RMSD bond angle | 3.422 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 26541 | |
<I/σ(I)> | 22.8 | |
Completeness [%] | 75.0 | 75 |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 277 | 12-14% PEG 40,000 1mM BTP pH 6.0, 200mM NaCl, EVAPORATION, temperature 277K, pH 6.00 |