3C6H
Crystal Structure of the RB49 gp17 nuclease domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.436, 126.724, 52.391 |
| Unit cell angles | 90.00, 106.03, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.800 |
| R-factor | 0.242 |
| Rwork | 0.242 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c6a |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.041 | 0.139 |
| Number of reflections | 29056 | |
| <I/σ(I)> | 18.6 | 8.7 |
| Completeness [%] | 95.0 | 86.9 |
| Redundancy | 3.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 15% PEG400, 0.2M KCl, 50mM Tris pH 8.5, 10mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
