3C6F
Crystal structure of protein Bsu07140 from Bacillus subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2006-10-11 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9796 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.268, 71.334, 70.094 |
Unit cell angles | 90.00, 111.93, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.2346 |
Rwork | 0.233 |
R-free | 0.28763 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.108 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.210 | 2.210 |
Rmerge | 0.060 | 0.770 |
Number of reflections | 31443 | |
<I/σ(I)> | 5.7 | 0.4 |
Completeness [%] | 96.1 | 89.2 |
Redundancy | 1.8 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 50mM Bis-Tris pH 6.5, 25% Pentaerythriol ethoxylate, 100 mM Ammonium sulfate, 10% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 294K |