3C65
Crystal Structure of Bacillus stearothermophilus UvrC 5' endonuclease domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Collection date | 2006-08-16 |
| Wavelength(s) | 0.97927, 0.97941 |
| Spacegroup name | P 61 |
| Unit cell lengths | 81.779, 81.779, 58.583 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| Rwork | 0.204 |
| R-free | 0.22100 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.259 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLOMON |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.076 | 0.251 |
| Number of reflections | 17709 | |
| <I/σ(I)> | 9.9 | |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 11.2 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop vapor diffusion | 8 | 292 | Tris-HCl, sodium citrate, sodium acetate, sodium formate, sodium chloride, pH 8.0, hanging drop vapor diffusion, temperature 292K |
