3C5Q
Crystal structure of diaminopimelate decarboxylase (I148L mutant) from Helicobacter pylori complexed with L-lysine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-12-29 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 79.111, 79.111, 134.821 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 14.980 - 2.400 |
Rwork | 0.202 |
R-free | 0.25300 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.304 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.140 | 0.373 |
Total number of observations | 14447 | |
Number of reflections | 19223 | |
<I/σ(I)> | 4.9 | 1.9 |
Completeness [%] | 98.2 | 99.9 |
Redundancy | 7.33 | 7.45 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1M Bicine, 1.6M Ammonium Sulfate, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |