3C5O
Crystal structure of the conserved protein of unknown function RPA1785 from Rhodopseudomonas palustris
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.117, 93.248, 111.340 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.890 - 2.200 |
R-factor | 0.216 |
Rwork | 0.213 |
R-free | 0.27100 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.367 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 29476 | |
<I/σ(I)> | 11 | 6.42 |
Completeness [%] | 87.4 | 58.6 |
Redundancy | 14.2 | 10.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.2M Mg Chloride, 0.1M Tris-HCl pH 8.5, 30% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 295K |