3C4X

Crystal Structure of G protein coupled receptor kinase 1 bound to ATP and magnesium chloride at 2.9A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-08-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97967
Spacegroup name	P 21 21 21
Unit cell lengths	54.116, 95.122, 234.823
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.750 - 2.900
R-factor	0.19307
Rwork	0.193
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3c4w
RMSD bond length	0.010
RMSD bond angle	1.228
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.900
Number of reflections	26629

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.3	277	PEG 3350, NaCl, glycerol, sodium citrate pH 4.3, ATP, magnesium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 277K