3C4U
Structure of class II fructose-biphosphate aldolase from helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.023, 82.560, 91.272 |
| Unit cell angles | 90.00, 99.66, 90.00 |
Refinement procedure
| Resolution | 33.404 - 1.830 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.20700 |
| Starting model (for MR) | 1rvg |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.573 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.101 | |
| Number of reflections | 71999 | |
| <I/σ(I)> | 18.5 | 6.4 |
| Completeness [%] | 99.4 | 0.983 |
| Redundancy | 8.64 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 297 | pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
