3C4S
Crystal structure of the Ssl0352 protein from Synechocystis sp. Northeast Structural Genomics Consortium target SgR42
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-01-25 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.97900 |
Spacegroup name | P 32 |
Unit cell lengths | 44.606, 44.606, 65.100 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.890 - 1.700 |
R-factor | 0.186 |
Rwork | 0.186 |
R-free | 0.19400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jz2 chain A |
RMSD bond length | 0.004 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.057 | 0.145 |
Number of reflections | 31765 | |
<I/σ(I)> | 17.36 | 6 |
Completeness [%] | 99.3 | 100 |
Redundancy | 3.9 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH UNDER OIL | 6.5 | 291 | 30% Jeffamine M-600, 0.05M Cesium chloride, 0.1M MES pH 6.5, MICROBATCH UNDER OIL, temperature 291K |