3C4S
Crystal structure of the Ssl0352 protein from Synechocystis sp. Northeast Structural Genomics Consortium target SgR42
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-01-25 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 32 |
| Unit cell lengths | 44.606, 44.606, 65.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.890 - 1.700 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jz2 chain A |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.057 | 0.145 |
| Number of reflections | 31765 | |
| <I/σ(I)> | 17.36 | 6 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH UNDER OIL | 6.5 | 291 | 30% Jeffamine M-600, 0.05M Cesium chloride, 0.1M MES pH 6.5, MICROBATCH UNDER OIL, temperature 291K |
