3C4R
Crystal structure of an uncharacterized protein encoded by cryptic prophage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-02 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.826, 106.570, 88.251 |
| Unit cell angles | 90.00, 96.42, 90.00 |
Refinement procedure
| Resolution | 31.090 - 2.300 |
| R-factor | 0.242 |
| Rwork | 0.242 |
| R-free | 0.28200 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.107 | 0.394 |
| Number of reflections | 26286 | |
| <I/σ(I)> | 20.8 | 1 |
| Completeness [%] | 88.2 | 60 |
| Redundancy | 6.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.1M Sodium cacodylate pH 5.5, 0.2M Magnesium acetate, 10% PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
