3C4N
Crystal structure of DR_0571 protein from Deinococcus radiodurans in complex with ADP. Northeast Structural Genomics Consortium Target DrR125
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.627, 67.829, 83.645 |
| Unit cell angles | 90.00, 91.14, 90.00 |
Refinement procedure
| Resolution | 19.980 - 2.400 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.24500 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.076 | 0.163 |
| Number of reflections | 65421 | |
| <I/σ(I)> | 10 | 3.64 |
| Completeness [%] | 90.8 | 97.5 |
| Redundancy | 1.6 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH UNDER OIL | 7.5 | 277 | Protein solution: 10 mM Tris-HCl pH 7.5, 100 mM NaCl, 5 mM DTT. Precipitant cocktail: 20% PEG 20000, 100 mM Lithium chloride, 100 mM Sodium acetate, MICROBATCH UNDER OIL, temperature 277K |
