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3C48

Structure of the retaining glycosyltransferase MshA: The first step in mycothiol biosynthesis. Organism: Corynebacterium glutamicum- APO (OPEN) structure.

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU RUH3R
Temperature [K]93
Detector technologyIMAGE PLATE
Collection date2007-08-01
DetectorRIGAKU RAXIS IV++
Wavelength(s)1.5418
Spacegroup nameP 31
Unit cell lengths79.743, 79.743, 148.364
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution34.530 - 2.100
R-factor0.18222
Rwork0.180
R-free0.22173
Structure solution methodSAD
RMSD bond length0.016
RMSD bond angle1.521
Data reduction softwareMOSFLM
Data scaling softwareSCALA
Phasing softwareSOLVE
Refinement softwareREFMAC (5.2.0019)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]35.0002.210
High resolution limit [Å]2.1002.100
Rmerge0.0520.238
Number of reflections60789
<I/σ(I)>16.52.9
Completeness [%]98.791.5
Redundancy2.92.5
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION8.5291Protein (15 mg/ml, 400 mM Ammonium sulfate, 10% glycerol, 0.5 mM EDTA, 1 mM BME): Precipitant (20% Peg4000, 100 mM Tris pH 8.5, 200 mm LiSO4), Vapor diffusion under oil, temperature 291K, VAPOR DIFFUSION

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