3C48
Structure of the retaining glycosyltransferase MshA: The first step in mycothiol biosynthesis. Organism: Corynebacterium glutamicum- APO (OPEN) structure.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 2007-08-01 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 |
Unit cell lengths | 79.743, 79.743, 148.364 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.530 - 2.100 |
R-factor | 0.18222 |
Rwork | 0.180 |
R-free | 0.22173 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.521 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.052 | 0.238 |
Number of reflections | 60789 | |
<I/σ(I)> | 16.5 | 2.9 |
Completeness [%] | 98.7 | 91.5 |
Redundancy | 2.9 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 291 | Protein (15 mg/ml, 400 mM Ammonium sulfate, 10% glycerol, 0.5 mM EDTA, 1 mM BME): Precipitant (20% Peg4000, 100 mM Tris pH 8.5, 200 mm LiSO4), Vapor diffusion under oil, temperature 291K, VAPOR DIFFUSION |