3C44
Crystal structure of HIV-1 subtype F DIS extended duplex RNA bound to paromomycin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2004-07-22 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9195 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 30.818, 95.703, 47.856 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.550 - 2.000 |
R-factor | 0.224 |
Rwork | 0.224 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3c3z |
RMSD bond length | 0.005 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 9333 | |
<I/σ(I)> | 25.7 | 6.2 |
Completeness [%] | 92.0 | 77.1 |
Redundancy | 3.7 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 310 | MPD, MgCl2, KCl, Na cacodylate, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 310K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Na cacodylate | ||
2 | 1 | 1 | MgCl2 | ||
3 | 1 | 1 | KCl | ||
4 | 1 | 1 | MPD | ||
5 | 1 | 2 | Na cacodylate | ||
6 | 1 | 2 | MgCl2 | ||
7 | 1 | 2 | KCl | ||
8 | 1 | 2 | MPD |