3C3P
Crystal structure of a methyltransferase (NP_951602.1) from Geobacter sulfurreducens at 1.90 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-20 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.00000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 156.880, 76.044, 85.173 |
Unit cell angles | 90.00, 118.27, 90.00 |
Refinement procedure
Resolution | 28.318 - 1.900 |
R-factor | 0.201 |
Rwork | 0.199 |
R-free | 0.23200 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.867 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.318 | 28.318 | 1.950 |
High resolution limit [Å] | 1.900 | 8.500 | 1.900 |
Rmerge | 0.056 | 0.030 | 0.507 |
Total number of observations | 2367 | 19337 | |
Number of reflections | 69499 | ||
<I/σ(I)> | 7.8 | 22.4 | 1.3 |
Completeness [%] | 99.9 | 88.2 | 100 |
Redundancy | 3.8 | 3.3 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 10.3 | 277 | NANODROP, 0.2M Sodium chloride, 18.0% PEG 8000, 0.1M CAPS pH 10.3, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
2 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 277 | NANODROP, 0.2M Sodium chloride, 20.0% PEG 8000, 0.1M CAPS pH 10.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |