3C3L
X-ray crystal structure of the N4 mini-vRNAP P2 promoter complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-09-16 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5414 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 82.344, 111.205, 276.954 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| Rwork | 0.215 |
| R-free | 0.24600 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.238 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 5.160 | 2.400 |
| Rmerge | 0.064 | 0.032 | 0.184 |
| Number of reflections | 92251 | ||
| <I/σ(I)> | 27.4 | ||
| Completeness [%] | 93.1 | 90.1 | 83.7 |
| Redundancy | 3.9 | 4.9 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 0.1 M Tris-HCl (pH 8.0), 0.3 M dibasic ammonium citrate, 25 % (drop) or 15% (reservoir) PEG 3350, hanging drop, temperature 294K, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Tris-HCl | ||
| 2 | 1 | 1 | dibasic ammonium citrate | ||
| 3 | 1 | 1 | PEG 3350 | ||
| 4 | 1 | 2 | Tris-HCl | ||
| 5 | 1 | 2 | dibasic ammonium citrate | ||
| 6 | 1 | 2 | PEG 3350 |
