3C3K
Crystal structure of an uncharacterized protein from Actinobacillus succinogenes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-01-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97900 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.888, 67.602, 204.802 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.470 - 1.990 |
R-factor | 0.183 |
Rwork | 0.180 |
R-free | 0.23100 |
Structure solution method | SAD |
RMSD bond length | 0.022 |
RMSD bond angle | 1.994 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | RESOLVE (2.13) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.060 |
High resolution limit [Å] | 1.990 | 4.290 | 1.990 |
Rmerge | 0.088 | 0.048 | 0.245 |
Number of reflections | 55962 | ||
<I/σ(I)> | 8.4 | ||
Completeness [%] | 86.4 | 99.7 | 38.4 |
Redundancy | 3 | 3.6 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 294 | 0.2 M Magnesium chloride, 0.1 M Bis-tris, 25% PEG 3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |