3C3D
Crystal structure of 2-phospho-(S)-lactate transferase from Methanosarcina mazei in complex with Fo and phosphate. Northeast Structural Genomics Consortium target MaR46
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-09 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97916 |
Spacegroup name | P 32 |
Unit cell lengths | 185.278, 185.278, 67.789 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.590 - 2.500 |
R-factor | 0.195 |
Rwork | 0.195 |
R-free | 0.23100 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.590 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.088 | 0.410 |
Number of reflections | 178601 | |
<I/σ(I)> | 15.37 | 4.15 |
Completeness [%] | 99.1 | 98 |
Redundancy | 5.1 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | Protein solution: 10 mM Tris-HCl pH 7.5, 100 mM NaCl, 5 mM Fo, 5 mM Lactyl phosphate, 5 mM DTT. Reservoir solution: 4.2 M Sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |