3C3A
Crystal Structure of human phosphoglycerate kinase bound to 3-phosphoglycerate and L-ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.629, 55.949, 95.441 |
| Unit cell angles | 101.88, 95.20, 82.91 |
Refinement procedure
| Resolution | 43.200 - 2.300 |
| R-factor | 0.18084 |
| Rwork | 0.179 |
| R-free | 0.27789 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vjc |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.630 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.4.0062) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.640 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.081 | 0.318 |
| Number of reflections | 26390 | |
| <I/σ(I)> | 9.3 | 2.7 |
| Completeness [%] | 84.9 | 55.3 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 291 | 2.6M NaKPO4, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
