3C38

Crystal structure of the periplasmic domain of Vibrio Cholerae LuxQ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	298
Detector technology	CCD
Collection date	2004-03-10
Wavelength(s)	0.97951
Spacegroup name	P 41 21 2
Unit cell lengths	55.656, 55.656, 161.415
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.770 - 2.300
R-factor	0.243
Rwork	0.243
R-free	0.26000
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	31.800
High resolution limit [Å]	2.300
Number of reflections	11949

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	12% v/v PEG 20000, 0.1M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K