3C37
X-ray structure of the putative Zn-dependent peptidase Q74D82 at the resolution 1.7 A. Northeast Structural Genomics Consortium target GsR143A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-11 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97900 |
Spacegroup name | P 1 |
Unit cell lengths | 36.840, 44.613, 71.772 |
Unit cell angles | 88.07, 75.18, 65.64 |
Refinement procedure
Resolution | 27.430 - 1.700 |
R-factor | 0.22106 |
Rwork | 0.219 |
R-free | 0.25386 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.238 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 84304 | |
<I/σ(I)> | 30.2 | 3.9 |
Completeness [%] | 94.7 | 86.3 |
Redundancy | 1.92 | 1.93 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Ammonium thiocyanate, 0.1M Sodium citrate, 40% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K | |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Ammonium thiocyanate, 0.1M Sodium citrate, 40% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K | |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Ammonium thiocyanate, 0.1M Sodium citrate, 40% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |