3C36

Crystal structure of GluR5 ligand-binding core in complex with ammonium ions at 1.68 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-14
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00000
Spacegroup name	P 41 21 2
Unit cell lengths	70.760, 70.760, 234.323
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.070 - 1.680
R-factor	0.16858
Rwork	0.167
R-free	0.20060
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3c31
RMSD bond length	0.019
RMSD bond angle	1.765
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.4.0066)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.760
High resolution limit [Å]	1.680	1.680
Rmerge	0.065	0.445
Number of reflections	68619
<I/σ(I)>	16.3	4.5
Completeness [%]	99.3	99.6
Redundancy	6	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	20% PEG 4000, 125 mM NH4Cl, 175 mM (NH4)2(SO4), 100 mM NH4 cacodylate, 4 mM Kainic acid, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K