3C34
Crystal structure of GluR5 ligand-binding core in complex with rubidium at 1.82 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-14 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 70.952, 70.952, 234.326 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.100 - 1.820 |
R-factor | 0.16848 |
Rwork | 0.166 |
R-free | 0.20897 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3c31 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.653 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.890 |
High resolution limit [Å] | 1.820 | 1.820 |
Rmerge | 0.075 | 0.423 |
Number of reflections | 54555 | |
<I/σ(I)> | 13.9 | 5.22 |
Completeness [%] | 99.5 | 100 |
Redundancy | 6.3 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 20% PEG 4000, 125 mM RbCl, 175 mM Rb2(SO4), 100 mM Rb cacodylate, 4 mM Kainic acid, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |