3C30
Crystal structure of the Vibrio Cholerae LuxQ periplasmic domain (SeMet)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2003-02-19 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97914 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 55.584, 55.584, 160.959 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.860 - 2.800 |
R-factor | 0.201 |
Rwork | 0.201 |
R-free | 0.22800 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 27.860 |
High resolution limit [Å] | 2.800 |
Number of reflections | 11874 |
<I/σ(I)> | 21.8 |
Completeness [%] | 99.8 |
Redundancy | 12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 12% v/v PEG 20000, 0.1M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |