3C2P
X-ray crystal structure of the N4 mini-vRNAP P1 promoter complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-29 |
Detector | ADSC Q315 |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.463, 111.194, 276.870 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
Rwork | 0.220 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
Rmerge | 0.093 | 0.037 | 0.578 |
Number of reflections | 151716 | ||
<I/σ(I)> | 8.8 | ||
Completeness [%] | 89.7 | 86 | 86.8 |
Redundancy | 3 | 3.2 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 0.1 M Tris-HCl (pH 8.0), 0.3 M dibasic ammonium citrate, 25 % (drop) or 15% (reservoir) PEG 6000, hanging drop, temperature 294K, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Tris-HCl | ||
2 | 1 | 1 | dibasic ammonium citrate | ||
3 | 1 | 1 | PEG 6000 | ||
4 | 1 | 2 | Tris-HCl | ||
5 | 1 | 2 | dibasic ammonium citrate | ||
6 | 1 | 2 | PEG 6000 |