3C29
Cre-loxP Synaptic structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 136.413, 136.413, 218.826 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.130 - 2.200 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.22800 |
| Starting model (for MR) | 1crx |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.555 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 119629 | |
| <I/σ(I)> | 5 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.2 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | MPD, Acetate, Calcium Chloride, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | Acetate | ||
| 3 | 1 | 1 | Calcium Chloride | ||
| 4 | 1 | 2 | MPD | ||
| 5 | 1 | 2 | Acetate | ||
| 6 | 1 | 2 | Calcium Chloride |
