3C28
Crystal structure of the product synapse complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 108.254, 122.453, 178.991 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.100 - 2.600 |
| R-factor | 0.228 |
| Rwork | 0.225 |
| R-free | 0.29200 |
| Starting model (for MR) | 1crx |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.364 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.073 | 0.323 |
| Number of reflections | 41399 | |
| <I/σ(I)> | 14.2 | |
| Completeness [%] | 99.8 | 98.1 |
| Redundancy | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | MPD, SODIUM ACETATE, CALCIUM Chloride, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | SODIUM ACETATE | ||
| 3 | 1 | 1 | CALCIUM Chloride | ||
| 4 | 1 | 2 | MPD | ||
| 5 | 1 | 2 | SODIUM ACETATE | ||
| 6 | 1 | 2 | CALCIUM Chloride |
