3C1Q

The three-dimensional structure of the cytoplasmic domains of EpsF from the Type 2 Secretion System of Vibrio cholerae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-30
Detector	RIGAKU SATURN 944
Spacegroup name	P 21 21 21
Unit cell lengths	46.988, 52.122, 89.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.940 - 1.700
R-factor	0.15846
Rwork	0.156
R-free	0.20793
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vma 2vmb
RMSD bond length	0.012
RMSD bond angle	1.274
Data reduction software	CrystalClear
Data scaling software	CrystalClear
Phasing software	MOLREP
Refinement software	REFMAC (5.3.0021)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.050	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.060	0.223
Number of reflections	23838
<I/σ(I)>	15	3.3
Completeness [%]	95.6	79.1
Redundancy	3.78	1.97

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	1ul protein at 10mg/ml + 1ul reservoir: 12.5% PEG 400, 200mM CaOAc2, 100mM MES pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K