3C1C

The effect of H3 K79 dimethylation and H4 K20 trimethylation on nucleosome and chromatin structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	292
Detector technology	CCD
Collection date	2006-09-07
Spacegroup name	P 21 21 21
Unit cell lengths	107.030, 110.080, 182.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.000 - 3.150
Rwork	0.220
R-free	0.29000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1aoi
RMSD bond length	0.008
RMSD bond angle	1.382
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.000	3.260
High resolution limit [Å]	3.150	3.150
Rmerge	0.088	0.285
Number of reflections	34295
<I/σ(I)>	10.7	3.8
Completeness [%]	90.0	90

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	292	Manganese chloride, Potassium chloride, Potassium cacodylate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	Manganese chloride
2	1	1	Potassium cacodylate
3	1	2	Potassium cacodylate
4	1	2	Potassium chloride