3C18
Crystal structure of nucleotidyltransferase-like protein (ZP_00538802.1) from Exiguobacterium sibiricum 255-15 at 1.90 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-12-05 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91837, 0.97951 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 66.740, 66.740, 456.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.709 - 1.900 |
R-factor | 0.186 |
Rwork | 0.183 |
R-free | 0.23300 |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.294 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SOLVE |
Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.709 | 29.709 | 1.970 |
High resolution limit [Å] | 1.900 | 4.080 | 1.900 |
Rmerge | 0.068 | 0.034 | 0.534 |
Number of reflections | 95241 | 18040 | 18305 |
<I/σ(I)> | 9.14 | 23.1 | 2.2 |
Completeness [%] | 99.9 | 99.1 | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.17 | 277 | NANODROP, 10.0% PEG 6000, 0.9M Lithium chloride, 0.1M Tris-HCl pH 8.17, VAPOR DIFFUSION, SITTING DROP, temperature 277K |