3C12
Crystal Structure of FlgD from Xanthomonas campestris: Insights into the Hook Capping Essential for Flagellar Assembly
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL12B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL12B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 116.710, 116.710, 71.820 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.270 - 2.510 |
| R-factor | 0.257 |
| Rwork | 0.257 |
| R-free | 0.27200 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.540 |
| Rmerge | 0.056 | 0.480 |
| Number of reflections | 11527 | |
| <I/σ(I)> | 33 | 3.3 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 11 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 295 | VAPOR DIFFUSION, temperature 295K |
