3C0T
Structure of the Schizosaccharomyces pombe Mediator subcomplex Med8C/18
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 111.016, 111.016, 68.640 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.400 |
Rwork | 0.211 |
R-free | 0.23140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | The starting model is the structure of Med18 alone which has not been deposited. |
RMSD bond length | 0.006 |
RMSD bond angle | 1.293 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 19282 | |
<I/σ(I)> | 21.4 | 4.8 |
Completeness [%] | 99.4 | 99.4 |
Redundancy | 11 | 11.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 100mM Tris pH 8.5, 2M sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |