3C0P
Maize cytokinin oxidase/dehydrogenase complexed with the allenic cytokinin analog HA-8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-17 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 250.700, 50.200, 51.200 |
| Unit cell angles | 90.00, 93.80, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.950 |
| Rwork | 0.215 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w1o |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.260 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.050 | |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 44993 | |
| <I/σ(I)> | 26.6 | 11.9 |
| Completeness [%] | 96.3 | 99.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 26% (w/v) PEG 1500, 0.5% (w/v) n-octyl beta-D glucoside, 0.1 M Tris-HCl, 1.5 mM HA-8, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
