3C0P
Maize cytokinin oxidase/dehydrogenase complexed with the allenic cytokinin analog HA-8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-17 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.934 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 250.700, 50.200, 51.200 |
Unit cell angles | 90.00, 93.80, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.950 |
Rwork | 0.215 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1w1o |
RMSD bond length | 0.006 |
RMSD bond angle | 1.260 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 2.050 | |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 44993 | |
<I/σ(I)> | 26.6 | 11.9 |
Completeness [%] | 96.3 | 99.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 26% (w/v) PEG 1500, 0.5% (w/v) n-octyl beta-D glucoside, 0.1 M Tris-HCl, 1.5 mM HA-8, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |