3C0J
Structure of E. coli dihydrodipicolinate synthase complexed with hydroxypyruvate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 120.967, 120.967, 110.639 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.490 - 2.400 |
| R-factor | 0.1734 |
| Rwork | 0.170 |
| R-free | 0.23729 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yxc |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.765 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.790 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.114 | 0.393 |
| Number of reflections | 36400 | |
| <I/σ(I)> | 7.8 | 2.8 |
| Completeness [%] | 98.5 | 98.9 |
| Redundancy | 3.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10 | 285 | 1.8M K2HPO4 (pH 10.0), 6% w/v N-octyl-D-glucopyranoside, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
