3C0C
X-ray Crystal Structure of the Rat Endophilin A2 SH3 Domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9000, 0.9795, 0.9798 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 64.970, 64.970, 41.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.700 |
| R-factor | 0.197 |
| Rwork | 0.196 |
| R-free | 0.21700 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.400 |
| Data scaling software | d*TREK (8.0L) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 34.890 | 34.890 | 1.460 |
| High resolution limit [Å] | 1.410 | 3.040 | 1.410 |
| Rmerge | 0.081 | 0.039 | 0.620 |
| Total number of observations | 21718 | 280 | |
| Number of reflections | 13188 | ||
| <I/σ(I)> | 11 | 32.4 | 0.7 |
| Completeness [%] | 75.2 | 88.3 | 13.3 |
| Redundancy | 9.04 | 12.81 | 1.23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop vapor diffusion | 6.5 | 277 | 1.9 M magnesium sulfate, 0.1 M MES, pH 6.5, hanging drop vapor diffusion, temperature 277K |
