3C0C

X-ray Crystal Structure of the Rat Endophilin A2 SH3 Domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9000, 0.9795, 0.9798
Spacegroup name	P 43 21 2
Unit cell lengths	64.970, 64.970, 41.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.700
R-factor	0.197
Rwork	0.196
R-free	0.21700
RMSD bond length	0.012
RMSD bond angle	1.400
Data scaling software	d*TREK (8.0L)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.890	34.890	1.460
High resolution limit [Å]	1.410	3.040	1.410
Rmerge	0.081	0.039	0.620
Total number of observations		21718	280
Number of reflections	13188
<I/σ(I)>	11	32.4	0.7
Completeness [%]	75.2	88.3	13.3
Redundancy	9.04	12.81	1.23

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop vapor diffusion	6.5	277	1.9 M magnesium sulfate, 0.1 M MES, pH 6.5, hanging drop vapor diffusion, temperature 277K