3C00
Crystal structural of the mutated G247T EscU/SpaS C-terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-01-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 26.969, 53.884, 59.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.410 |
R-factor | 0.217 |
Rwork | 0.216 |
R-free | 0.23600 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.503 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.470 |
High resolution limit [Å] | 1.410 | 3.060 | 1.410 |
Rmerge | 0.057 | 0.038 | 0.695 |
Number of reflections | 17142 | ||
<I/σ(I)> | 11.4 | ||
Completeness [%] | 99.4 | 94.4 | 99.9 |
Redundancy | 12.9 | 12.3 | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7.5 | 293 | Tris, HEPES, Peg3350, NaCl, pH 7.5, Microbatch, temperature 293K |