3BZV
Crystal structural of the mutated T264A EscU C-terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-01-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 52.086, 52.086, 155.184 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.130 - 1.940 |
R-factor | 0.217 |
Rwork | 0.215 |
R-free | 0.26800 |
RMSD bond length | 0.023 |
RMSD bond angle | 2.108 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.010 |
High resolution limit [Å] | 1.940 | 4.180 | 1.940 |
Rmerge | 0.066 | 0.051 | 0.725 |
Number of reflections | 9941 | ||
<I/σ(I)> | 10.5 | ||
Completeness [%] | 99.6 | 99.2 | 97.4 |
Redundancy | 13 | 11 | 11.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5.5 | 293 | Li2SO4, Bis-Tris, Peg3350, NaCl, pH 5.5, Microbatch, temperature 293K |