3BZT

Crystal structural of the mutated P263A EscU C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-01-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97956
Spacegroup name	I 2 2 2
Unit cell lengths	56.411, 59.091, 72.105
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.690 - 1.500
R-factor	0.185
Rwork	0.184
R-free	0.20500
RMSD bond length	0.012
RMSD bond angle	1.394
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.550
High resolution limit [Å]	1.500	3.230	1.500
Rmerge	0.057	0.042	0.451
Number of reflections	19406
<I/σ(I)>	13.6
Completeness [%]	98.5	94.5	96.4
Redundancy	6.5	6	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7	293	(NH4)3 Citrate, NaCl, pH 7.0, Microbatch, temperature 293K