3BZP
Crystal structural of the mutated N262A EscU C-terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-01-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97956 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 56.535, 59.169, 71.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.690 - 1.499 |
R-factor | 0.19456 |
Rwork | 0.194 |
R-free | 0.21300 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.634 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.550 |
High resolution limit [Å] | 1.499 | 3.230 | 1.499 |
Rmerge | 0.051 | 0.035 | 0.400 |
Number of reflections | 18747 | ||
<I/σ(I)> | 12.8 | ||
Completeness [%] | 94.9 | 81.9 | 90.4 |
Redundancy | 7 | 6.5 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5.5 | 293 | Citrate, PEG 6000, t-Butanol, pH 5.5, Microbatch, temperature 293K |