3BZO
Crystal structural of native EscU C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-30 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97945 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 55.692, 58.708, 72.605 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.640 - 1.500 |
| R-factor | 0.203 |
| Rwork | 0.202 |
| R-free | 0.22500 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.289 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 3.230 | 1.500 |
| Rmerge | 0.047 | 0.032 | 0.538 |
| Number of reflections | 19133 | ||
| <I/σ(I)> | 16.8 | ||
| Completeness [%] | 97.8 | 98.2 | 95.7 |
| Redundancy | 11.8 | 11.4 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | K2SO4, PEG 3350, pH 8.0, vapor diffusion, temperature 293K |
