3BZF
The human non-classical major histocompatibility complex molecule HLA-E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.520, 62.301, 98.769 |
| Unit cell angles | 90.00, 106.15, 90.00 |
Refinement procedure
| Resolution | 29.600 - 2.500 |
| R-factor | 0.20374 |
| Rwork | 0.200 |
| R-free | 0.26936 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mhe |
| RMSD bond length | 0.034 |
| RMSD bond angle | 2.354 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.565 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.098 | 0.344 |
| Number of reflections | 32033 | |
| <I/σ(I)> | 12.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 293 | 19% PEG 3350, 0.3M NaCl, 0.1M Tris, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
